rabbit polyclonal anti v5 cell signaling Search Results


anti v5 horseradish peroxidase  (Thermo Fisher)
99
Thermo Fisher anti v5 horseradish peroxidase
Anti V5 Horseradish Peroxidase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 99 stars, based on 1 article reviews
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rabbit anti v5  (Cell Signaling Technology Inc)
98
Cell Signaling Technology Inc rabbit anti v5
Rabbit Anti V5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 98 stars, based on 1 article reviews
rabbit anti v5 - by Bioz Stars, 2026-02
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ab1229  (Abcam)
95
Abcam ab1229
Ab1229, supplied by Abcam, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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anti-v5  (Cell Signaling Technology Inc)
90
Cell Signaling Technology Inc anti-v5
Anti V5, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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a190 120a  (Bethyl)
95
Bethyl a190 120a
A190 120a, supplied by Bethyl, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 95 stars, based on 1 article reviews
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mouse anti-v5 serum  (Bethyl)
90
Bethyl mouse anti-v5 serum
Mouse Anti V5 Serum, supplied by Bethyl, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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mouse monoclonal anti-v5 epitope antibody  (Thermo Fisher)
90
Thermo Fisher mouse monoclonal anti-v5 epitope antibody
Mouse Monoclonal Anti V5 Epitope Antibody, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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anti-v5  (Bio-Rad)
90
Bio-Rad anti-v5
(A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of <t>V5-tagged</t> EIF3A in the different fractions was analysed by <t>western</t> <t>blotting.</t> (B) Same experiment as described in (A) , including cells lacking 4EIP.
Anti V5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
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rabbit v8137 anti-v5 polyclonal antibody  (Millipore)
90
Millipore rabbit v8137 anti-v5 polyclonal antibody
(A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of <t>V5-tagged</t> EIF3A in the different fractions was analysed by <t>western</t> <t>blotting.</t> (B) Same experiment as described in (A) , including cells lacking 4EIP.
Rabbit V8137 Anti V5 Polyclonal Antibody, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
rabbit v8137 anti-v5 polyclonal antibody - by Bioz Stars, 2026-02
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mouse anti v5  (Santa Cruz Biotechnology)
93
Santa Cruz Biotechnology mouse anti v5
(A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of <t>V5-tagged</t> EIF3A in the different fractions was analysed by <t>western</t> <t>blotting.</t> (B) Same experiment as described in (A) , including cells lacking 4EIP.
Mouse Anti V5, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti v5 af55  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc anti v5 af55
(A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of <t>V5-tagged</t> EIF3A in the different fractions was analysed by <t>western</t> <t>blotting.</t> (B) Same experiment as described in (A) , including cells lacking 4EIP.
Anti V5 Af55, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
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anti v5  (Bio-Rad)
96
Bio-Rad anti v5
(A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of <t>V5-tagged</t> EIF3A in the different fractions was analysed by <t>western</t> <t>blotting.</t> (B) Same experiment as described in (A) , including cells lacking 4EIP.
Anti V5, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


(A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of V5-tagged EIF3A in the different fractions was analysed by western blotting. (B) Same experiment as described in (A) , including cells lacking 4EIP.

Journal: bioRxiv

Article Title: Mechanisms of translation repression by the EIF4E1-4EIP cap-binding complex of Trypanosoma brucei : potential roles of the NOT complex and a terminal uridylyl transferase

doi: 10.1101/2021.05.19.444837

Figure Lengend Snippet: (A) PTP-tagged EIF4E1 was pulled down from 1 × 10 8 bloodstream forms (BSFs) of Trypanosoma brucei . Enrichment of V5-tagged EIF3A in the different fractions was analysed by western blotting. (B) Same experiment as described in (A) , including cells lacking 4EIP.

Article Snippet: The following antibodies were used for specific protein detection: anti-4E1 serum (1:2,000, rabbit; kind gift from Osvaldo de Melo Neto), anti-S9 serum (1:20,000, rat; loading control), anti-myc (1:5,000, mouse; Y2H), anti-p-GPEET (1:2,000, mouse, Cedarlane; western blotting), anti-V5 (1:2,000, mouse, Biorad; western blotting), anti-PAP (1:20,000, rabbit, Sigma; western blotting), anti-aldolase serum (1:50,000, rabbit; western blotting), anti-EP (1:2,000, mouse, Cedarlane; western blotting), and anti-CAT serum (1:2,000, rabbit), anti-rabbit IgG (pull-downs).

Techniques: Western Blot

(A) Pull-downs of V5-tagged 4EIP from bloodstream form (BSF) Trypanosoma brucei parasites in absence or presence of RNase A (left and right panels, respectively), followed by detection of TUT3-myc enrichment by western blotting. (B) Pull-down of myc-tagged TUT3 from bloodstream form T. brucei parasites, followed by detection of 4EIP-V5 by western blotting. (C, D) Two representative replicates of pull-downs of PTP-tagged EIF4E1 from bloodstream form T. brucei parasites, followed by detection of CAF1-V5 by western blotting.

Journal: bioRxiv

Article Title: Mechanisms of translation repression by the EIF4E1-4EIP cap-binding complex of Trypanosoma brucei : potential roles of the NOT complex and a terminal uridylyl transferase

doi: 10.1101/2021.05.19.444837

Figure Lengend Snippet: (A) Pull-downs of V5-tagged 4EIP from bloodstream form (BSF) Trypanosoma brucei parasites in absence or presence of RNase A (left and right panels, respectively), followed by detection of TUT3-myc enrichment by western blotting. (B) Pull-down of myc-tagged TUT3 from bloodstream form T. brucei parasites, followed by detection of 4EIP-V5 by western blotting. (C, D) Two representative replicates of pull-downs of PTP-tagged EIF4E1 from bloodstream form T. brucei parasites, followed by detection of CAF1-V5 by western blotting.

Article Snippet: The following antibodies were used for specific protein detection: anti-4E1 serum (1:2,000, rabbit; kind gift from Osvaldo de Melo Neto), anti-S9 serum (1:20,000, rat; loading control), anti-myc (1:5,000, mouse; Y2H), anti-p-GPEET (1:2,000, mouse, Cedarlane; western blotting), anti-V5 (1:2,000, mouse, Biorad; western blotting), anti-PAP (1:20,000, rabbit, Sigma; western blotting), anti-aldolase serum (1:50,000, rabbit; western blotting), anti-EP (1:2,000, mouse, Cedarlane; western blotting), and anti-CAT serum (1:2,000, rabbit), anti-rabbit IgG (pull-downs).

Techniques: Western Blot

(A) Differentiation-competent T. brucei (EATRO1125 strain) with one V5-tagged TUT3 gene and RNAi targeting TUT3 were grown to a maximum density of 2 × 10 6 cells/mL. 6 mM cis -aconitate (CA) was then added, and the temperature was reduced to 27 °C to induce differentiation. Cultures were grown with or without tetracycline, with the drug being included for 24 h before addition of cis -aconitate. For each time point, 5 × 10 6 cells were collected for western blot measurement of V5-TUT3, Ep procyclin, and a control rpotein, ribosomal protein S9. (B) Tethering of lambdaN-TUT3-myc has no effect on a box-B-containing reporter mRNA encoding chloramphenicaol acetyltransferase (CAT). Expression of lambdaN-TUT3-myc was induced for 24h then CATwas measured by Western blotting. A protein that cross-reacts with the anti-CAT antibody served as a loading control.

Journal: bioRxiv

Article Title: Mechanisms of translation repression by the EIF4E1-4EIP cap-binding complex of Trypanosoma brucei : potential roles of the NOT complex and a terminal uridylyl transferase

doi: 10.1101/2021.05.19.444837

Figure Lengend Snippet: (A) Differentiation-competent T. brucei (EATRO1125 strain) with one V5-tagged TUT3 gene and RNAi targeting TUT3 were grown to a maximum density of 2 × 10 6 cells/mL. 6 mM cis -aconitate (CA) was then added, and the temperature was reduced to 27 °C to induce differentiation. Cultures were grown with or without tetracycline, with the drug being included for 24 h before addition of cis -aconitate. For each time point, 5 × 10 6 cells were collected for western blot measurement of V5-TUT3, Ep procyclin, and a control rpotein, ribosomal protein S9. (B) Tethering of lambdaN-TUT3-myc has no effect on a box-B-containing reporter mRNA encoding chloramphenicaol acetyltransferase (CAT). Expression of lambdaN-TUT3-myc was induced for 24h then CATwas measured by Western blotting. A protein that cross-reacts with the anti-CAT antibody served as a loading control.

Article Snippet: The following antibodies were used for specific protein detection: anti-4E1 serum (1:2,000, rabbit; kind gift from Osvaldo de Melo Neto), anti-S9 serum (1:20,000, rat; loading control), anti-myc (1:5,000, mouse; Y2H), anti-p-GPEET (1:2,000, mouse, Cedarlane; western blotting), anti-V5 (1:2,000, mouse, Biorad; western blotting), anti-PAP (1:20,000, rabbit, Sigma; western blotting), anti-aldolase serum (1:50,000, rabbit; western blotting), anti-EP (1:2,000, mouse, Cedarlane; western blotting), and anti-CAT serum (1:2,000, rabbit), anti-rabbit IgG (pull-downs).

Techniques: Western Blot, Expressing